B Pharmacy 2nd Year Annual
Pharmaceutical Microbiology
BPH – 214
Time: Three Hours M. Marks : 80
Note: Attempt any five questions. All questions carry equal marks.
- What are micro-organisms? What are the various sites of the human body where they are normally distributed? Give a detailed classification of bacteria, virus and fungal pathogens.
- What are culture medias? Explain in detail the various types of medias used for the growth of bacteria. Also enlist the nutritional requirements needed in a culture media for the growth of bacteria.
- What are culture methods? Enlist the indications where they fin use. Explain in detail the method employed for isolating pure cultures of bacteria from mixtures.
- Explain in detail the process of fermentation taking into account the fermentation process required for production of citric acid and vitamin B12.
- Write notes on any two:(A) Sterility Testing(B) Preparation of BCG Vaccine(C) Microbiological assay of Penicillin
- Define sterilization. Give the classification of various agents used in sterilization. Explain about the various physical agents used in the process of sterilization.
- (A) Discuss about the various factors responsible for causing infection.(B) What are mutagenic agents? How they are isolated? Discuss the factors affecting rate of mutation.
- Differentiate between:(A) Exotoxins and Endotoxins(B) Active Immunity and Passive Immunity(C) Antigens and Antibodies(D) Vaccines and Sera