Pharmaceutical Microbiology
B. Pharmacy 2nd Year
BPH – 214
Time: Three Hours M. Marks : 80
Note: Attempt any five questions. All questions carry equal marks.
- (A) Describe the various methods for cultivation of animal viruses.(B) Describe a typical growth curve for a batch culture of bacteria and discuss its phases.
- (A) Describe the various nutritional requirements for growth and cultivation of bacteria and means/methods of supplying he requirements.(B) Discuss the nutritional requirements of fungi.
- (A) Describe any two methods for isolation of a particular bacterial species of interest.(B) Describe any two methods for “viable count” of bacteria.
- (A) Describe the mechanism of action in the following methods of sterilisation:(i) Dry Heat Sterilization(ii) Moist Heat Sterilization(iii) Ethylene Oxide Sterilization(iv) Sterilization by ionising radiation(B) Describe the method f Sterilization by filtration in details, giving examples of the various bacteria proof filters used. Discuss the application of this method.
- (A) Describe the Reidel Walker Coefficient test in datails. What are the limitations of this method?(B) Describe the method(s) for evaluation of bactaricidal and bacteriostatic activities of a pharmaceutical compound.
- (A) Describe the principle and method of microbiological assays of vitamins in general.(B) Describe the standardisation of bacterial vaccines.
- (A) Describe the construction and working of a batch type fermentor, using a diagram.(B) Describe the preparation and isolation of alcohol, by fermantation.
- (A) Differentiate between active immunity and passive immunity.(B) Name the various bacterial vaccines and describe the preparation of any one of them.(C) Discuss the tests for determining potency of bacterial vaccines.