PHARMACEUTICAL ANIMAL BIOTECHNOLOGY Theory Syllabus

BPH-308: PHARMACOLOGY – III

(PHARMACEUTICAL ANIMAL BIOTECHNOLOGY)

THEORY

Max. Marks: 80                                                                          Total Hours: 50 (2hrs/week)

Note: Examiner to set eight questions and the candidates are required to attempt any five.
1) Introduction to Biotechnology: Definitions, history, branches and scope of biotechnology. Therapeutic and pharmaceutical applications of biotechnology. (5)

2) DNA: Structure and function; DNA replication, repair and recombination, isolation of DNA; RAPD, RFLP and AFLP analysis. (7)
3) Genetic Engineering of Animal Cells: (9)

i. Introduction, gene transfer techniques, host system and gene cloning.
ii. Transgenic animals and their applications, characterization of transfected cells; in vitro fertilization, embryo transfer and ethical issues of animal cloning.

4) Enzymes: (6)

i. Introduction, mechanism of action, isolation, purification, immobilized enzymes, methods of immobilization, applications of immobilized enzymes.
ii. Study of enzymes such as hyaluronidase, streptokinase, amylase and protease.

5) Animal Tissue Culture: (8)

i. History and scope of tissue culture, tissue culture media;
ii. Sterilization of materials for tissue culture.
iii. Types of tissue culture, primary cultures and culture of cell lines,
iv. Characterization and preservation of cell lines, 3D culture techniques.

6) Stem cells and Gene Therapy: (6)

i. History and introduction to stem cells and gene therapy
ii. Human stem cell culture (embryonic and germ cells); methods of gene therapy;
iii. Applications of stem cells and gene therapy in pharmaceuticals, ethical issues related to stem cells.

7) Drug Delivery systems by Biotechnology: (9)

i. Production of drugs from biotechnology- erythropoietins, interferons, vaccines, anticoagulant agents, monoclonal antibodies and human and follicle stimulating growth hormone.
ii. Cancer immunotherapy vaccines
iii. Design of drug delivery systems for biotechnological products.

Books Recommended (Latest editions)

1. Balasubramanian, Bryce, Dharmalingam, Green and Jayaraman (Eds.), Concepts in Biotechnology, University Press.
2. Hood L.E., Weissman I., Wood W.B. and Wilson J.H. Immunology, Benjamin Cummings. 
3. Biotol Series – Butterworth and Heineman, Oxford, 1992.
4. Essential Immunology, Roitt, I.M., Blackwell Scientific, Oxford, UK. 
5. Immunology, Kuby, J., Freeman, W.H., Oxford. 
6. Biotechnology V, M. K. Sateesh. 
7. Culture of Animal Cells- I.Freshrey (Willy).

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