PHARMACEUTICAL MICROBIOLOGY Practical Syllabus

BPH-212: PHARMACEUTICS – IV

(PHARMACEUTICAL MICROBIOLOGY)

PRACTICAL

Max. Marks: 80                                                                               Total Hours: 75 (3hrs/week)

SUGGESTED EXPERIMENTS

1. Preparation and sterilization of aerobic and anaerobic media.

2. Aerobic and anaerobic cultivation of bacteria.

3. Gram’s staining, acid fast staining and hanging drop preparation.

4. Separation of mixed cultures and maintenance of pure cultures.

5. Microbial viable count in Pharmaceutical Formulations.

6. Particle count in water for injection.

7. Thermal death time studies.

8. Morphological characteristics of moulds and Yeasts.

9. Turbidimetric assay of at least one drug using microbial culture.

10. Bio-Chemistry reactions:

(a) Starch Hydrolysis

(b) Gelatin liquification and

(c) Haemolysis of blood

11. Phenol coefficient test for disinfectant.

12. Production of at least one product by fermentation.

13. Biological assay of tetracycline and cyanocobalamin.

14. Test for limit of alkalinity of glass.

15. Test for sterility. (Rabbit method and LAL method)

16. Test for Pyrogens.

17. Preparation of injections of water, dextrose, normal saline and oily phenol. Carrying out quality control tests.

NOTE: ANY OTHER EXPERIMENTS (S) MAY BE INCLUDED IN SUPPORT OF THE THEORETICAL ASPECTS OF THE COURSE.

Books Recommended (Latest edition):

1. J. Woodward (Editor), Immobilized Cells and Enzymes, A Practical Approach, IRL Press. 
2. L.M. Prescott, J.P. Harley and D.A. Klein, Microbiology, Wm. C. Brown Publishers, England. 
3. S. J. Carter, Cooper and Gunn’s Tutorial Pharmacy. Sixth Edition, pitman publishing Co, London. 
4. M. Furbisher, Fundamentals ofMicrobiology, 8th Edition, W. B. Saunders Company, Philadelphia, USA. 
5. W. B. Hugo and A. D. Russell, Pharmaceutical Microbiology, Blackwell Scientific publications, Oxford. 
6. Presscot & Donn, Industrial Microbiology, CBS. Publishers, Delhi. 
7. K. K. Kieslich (Editor). Biotechnology, Vol 6a, Verlag chemic, Basel, Switzerland. 
8. M. J. Pcelzar, Reid and chan, Microbiology, TATA Mc-Graw Hill publishing, Co. Ltd. New Delhi. 
9. S. J. Carter, Cooper and Gunn’s dispensing for Pharmaceutical students, 12th Edition, CBS Publishers & Distributors, 485, Bhola Nath Nager, Shahdara, Delhi-32. 
10. Osal, Remington’s Pharmaceutical Sciences, Mack publishing company, pennsylvania, U. S. A. 

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