PHARMACOGNOSY IV Practical Syllabus

BPH-414: PHARMACOGNOSY IV
PRACTICAL

Max. Marks: 80                                                                                 Total Hours: 75 (3hrs/week)

1. Laboratory design, Aseptic techniques, Media Preparation.
2. Initiation and maintenance of callus cultures.
3. Initiation and maintenance of plant cell suspension cultures.
4. Micro droplet and single cell nurse culture.
5. Culture organ explants and micro propagation by axillary bud proliferation and somatic embryogenesis.
6. Production of secondary metabolites Anthrocyanin pigments and Aromatic compounds.
7. Synthesis of artificial seeds and study of their delivery system.

NOTE: ANY OTHER EXPERIMENTS (S) MAY BE INCLUDED IN SUPPORT OF THE THEORETICAL ASPECTS OF THE COURSE.

Books Recommended (Latest editions)

1. Biotechnology and Pharmacy, John M, Pazzute, Michael E. Johnson, and Hanri R. Manasse, Jr.
2. An Introduction to plant Tissue culture by M. K. Razdan, 1994, Oxford & IEH.
3. Plant cell tissue and organ culture fundamental methods by O. L. Gambarg and G. C. Phillips, 1996, Narosa Publishing House.
4. Secondary metabolism in plnat cell cultures by phillip Marris, Alan H. Scragg, Angle Staff and Michael W. Fowlar 1986, Cambridge University Press.
5. Biotechnology in Agriculture and Forestry Vol. IV, Medicinal and arematic plants I by V. P. S. Bajaj 1988, Springer Verlag.
6. Biotechnological applications of plant cultures by peter D. Shargoel and That T. Ngo, 1994. CRC Press Inc.

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