PHARMACEUTICAL CHEMISTRY – V Practical Syllabus

BPH-211: PHARMACEUTICAL CHEMISTRY – V

PHARMACEUTICAL BIOCHEMISTRY

PRACTICAL

Max. Marks: 80                                                                                          Total Hours: 75 (3hrs/week)

1. Preparation of standard buffer (nitrate, phosphate, carbonate and measurement of pH).
2. Separation of lipids by TLC.
3. Quantitative estimation of amino acids.
4. Quantitative estimation of proteins.
5. Identification of C-terminal amino acids of proteins.
6. Estimation of blood glucose, blood cholesterol, SGPT and SGOT activity.
7. Enzymatic hydrolysis of glycogen by αand βamylase.
8. Acid hydrolysis and action of salivary amylase on starch.
9. Estimation of chloride, glucose, ammonia and creatinine in urine.
10. Identification of carbohydrates, proteins and fats.
11. Identification of abnormal constituents of urine.

NOTE: ANY OTHER EXPERIMENTS(S) MAY BE INCLUDED IN SUPPORT OF THE THEORETICAL ASPECTS OF THE COURSE.
Books Recommended (Latest edition):

1. D.W. Martin, PA Mays & V.M. Redwell, Harpers Review of Biochemistry, Lange Medical Publication (Latest Edition). 
2. RE. Conn & P.K. Stumpf, Outlines of Biochemistry, John Wiley & Sons, New York (Latest Edition). 
3. A.L. Lehninger, Biochemistry, Worth Publisher, Inc. (Latest Edition). 
4. A.L. Lehninger, Principles of Biochemistry, CBS Publishers and Distributors (Latest Edition). 
5. L. Stryer, Biochemistry, W.H. Freeman & Company, San Francisco (Latest Edition). 
6. Harrow & A. Mazur, Textbook of Biochemistry, W.B. Saunders Co., Philadelphia. (Latest Edition). 
7. M.N. Chatterjea & Rana Schinde, Textbook of Medical Biochemistry, Jintender P. Vij, New Delhi (Latest Edition).

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